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KMID : 0357820030270020056
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Korean Journal of Legal Medicine
2003 Volume.27 No. 2 p.56 ~ p.61
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Analysis of Five Coding Region Polymorphism in Mitochondrial DNA
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Zhang Yong-Ji
Lee Jung-Bin
Park Jin-Cheol
Zheng Zhe-Jia
Lin Hai-Yu
Kim So-Young
Choi Young-Tae
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Abstract
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For evaluation of the five coding region (CR) polymorphism in mitochondrial DNA (mtDNA); we had performed PCR and direct sequencing in 599 unrelated Korean who showed the identical DNA type in D-loop mitochondrial DNA analysis for total 2,810 bp fragment. Following the sequence analysis, all the sequences of five regions were compared respectively to Anderson standard sequence to investigate the nucleotide variations. The result showed, a total 4,565 nucleotide variations were observed at 190 positions in five CR as 3,931 (86.11%) substitutions, 32 (0.7%) insertions, and 602 (13.19%) deletions and the allele diversities (h) were higher than 0.9992 when adding each CR or combined CR to D-loop analysis in mtDNA. In conclusion, we could confirm the five CR are useful for forensic testing through the nucleotide variation and hapolotypes polymorphism.
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KEYWORD
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Coding Region, Polymorphism, PCR, Sequencing, Korean
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